Inactivation of a MAPK-like protein kinase and activation of a MBP kinase in germinating barley embryos
article
We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos. (C) 2000 Federation of European Biochemical Societies. Chemicals/CAS: Abscisic Acid, 21293-29-8; Ca(2+)-Calmodulin Dependent Protein Kinase, EC 2.7.1.123; fusicoccin, 20108-30-9; Glycogen Synthase Kinase 3, EC 2.7.1.37; Glycosides; Mitogen-Activated Protein Kinases, EC 2.7.1.37; Plant Growth Regulators; Water, 7732-18-5
TNO Identifier
56896
ISSN
00145793
Source
FEBS Letters, 484(1), pp. 55-59.
Pages
55-59