Expression of human lymphotoxin alpha in Aspergillus niger
article
A gene-fusion expression strategy was applied for heterologous expression of human lymphotoxin alpha (LTα) in the Aspergillus niger AB1.13 protease-deficient strain. The LTα gene was fused with the A. niger glucoamylase GII-form as a carrier-gene, behind its transcription control and secretion signals. Special attention was paid to the influence of different codon usage on secretion of protein. In the case of human tumor necrosis factor alpha (TNFα) a dramatic change of secretion has been observed when human cDNA sequence was used instead of synthetic E. coli biased codons. In the case of LTα such a change of codon usage brought improvement at the RNA level, however, no increase in the quantity of secreted protein was observed, due to the proteolitic activity of the host organism. The estimated yield of secretion of LTα from A. niger into the soya medium was 50 pg l-1 of culture. Chemicals/CAS: Glucan 1,4-alpha-Glucosidase, EC 3.2.1.3; Lymphotoxin-alpha; RNA, Messenger
Topics
MedicineGeneeskundePathologyAspergillus nigerCodon usageHeterologous protein secretionLTαTNFαComplementary DNALymphotoxinTumor necrosis factor alphaAspergillus nigerCodon usageConference paperControlled studyDNA sequenceGene controlGene fusionGenetic transcriptionHumanNonhumanPriority journalProtein degradationProtein secretionArtificial Gene FusionAspergillus nigerBlotting, NorthernGlucan 1,4-alpha-GlucosidaseHumansLymphotoxin-alphaRNA, Messenger
TNO Identifier
56828
ISSN
00316768
Source
Pflügers Archiv - European Journal of Physiology, 440(suppl. 5), pp. p. R83-R85.
Pages
p. R83-R85
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