Can hTNF-alpha be successfully produced and secreted in filamentous fungus Aspergillus niger?
article
A gene-fusion expression strategy was applied for the heterologous expression of hTNF-α in A. niger AB1.13. The TNF-α gene was fused with the A. niger glucoamylase GII form as a carrier-gene, behind its transcription control and secretion signal. The protein was expressed in the cells in the form of a glucoamylase-fusion protein, but was not present in the culture medium. From the expression of two hTNF-α analogues, LK 811 (Cys95/148) and LK 802 (Cys95/148, His107/108) respectively, we concluded that oligomerisation was not the critical point for secretion of hTNF-α in A. niger, but more probably improper folding already at the stage of monomer formation, or even incorrect processing of the molecule during the secretion pathway. © Springer Verlag 2000 Chemicals/CAS: Glucan 1,4-alpha-Glucosidase, EC 3.2.1.3; Tumor Necrosis Factor-alpha
Topics
Aspergillus nigerHeterologous protein secretionhTNF-αhTNF-α analoguesGlucan 1,4 alpha glucosidaseTumor necrosis factor alphaAmino acid sequenceAspergillus nigerBiosynthesisGene expressionGene fusionGeneticsHumanMetabolismSecretionAmino Acid SequenceArtificial Gene FusionAspergillus nigerGene ExpressionGlucan 1,4-alpha-GlucosidaseHumansTumor Necrosis Factor-alpha
TNO Identifier
56515
ISSN
00316768
Source
Pflügers Archiv - European Journal of Physiology, 439(7 suppl.), pp. p. R84-R86.
Pages
p. R84-R86
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