Expression of cbsA encoding the collagen-binding S-protein of Lactobacillus crispatus JCM5810 in Lactobacillus casei ATCC 393T
article
The cbsA gene encoding the collagen-binding S-layer protein of Lactobacillus crispatus JCM5810 was expressed in L. casei ATCC 393T. The S-protein was not retained on the surface of the recombinant bacteria but was secreted into the medium. By translational fusion of CbsA to the cell wall sorting signal of the proteinase, PrtP, of L. casei, CbsA was presented at the surface, rendering the transformants able to bind to immobilized collagens. Chemicals/CAS: Bacterial Proteins; Integrins; Membrane Glycoproteins; Membrane Proteins; Receptors, Collagen; surface array protein, bacteria
Topics
Bacterial proteinBinding proteinCollagen binding s proteinUnclassified drugBacterium adherenceGene expressionLactobacillusLactobacillus caseiLactobacillus crispatusNonhumanPriority journalProtein secretionSignal transductionBacterial ProteinsCell MembraneIntegrinsLactobacillusLactobacillus caseiMembrane GlycoproteinsMembrane ProteinsReceptors, CollagenBacteria (microorganisms)Lactobacillus caseiLactobacillus crispatusPosibacteria
TNO Identifier
41891
ISSN
00219193
Source
Journal of Bacteriology, 182(23), pp. 6857-6861.
Pages
6857-6861
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