Effects of vitamin C supplementation in human volunteers with a range of cholesterol levels on biomarkers of oxygen radical-generated damage
article
Twenty-four men and 24 women, all nonsmoking, and maintaining normal dietary habits were assigned to 3 groups of 16. Each group comprising 4 males with "low" cholesterol levels (<6 mmol/L) matched for age and build with 4 males with "high" cholesterol levels (>6 mmol/L) and 8 similarly matched females. A three-treatment, three-treatment period, cross-over design was adopted. The three treatments were placebo, 60 mg vitamin C/day (the recommended daily allowance) and 6 g vitamin C/day for 14 days with 6 weeks between treatments. Blood samples were taken at the end of each treatment period. Vitamin C supplementation significantly increased plasma vitamin C concentrations and total antioxidant capacity, but did not affect cholesterol status or plasma ras p21 protein levels. There was a nonsignificant dose-related decrease in plasma lipid peroxidation breakdown products. DNA damage, measured in lymphocytes by the Comet assay and chromosome aberration test, was not increased after vitamin C supplementation. Sensitivity to hydrogen peroxide (in the Comet assay) was also unaffected, but sensitivity to chromosome aberration induced by bleomycin was increased by supplementation. A significant gender difference was found in plasma vitamin C levels, antioxidant capacity, and number of chromosome aberrations. Results were independent of low and high cholesterol status.
Topics
MedicineGeneeskundeHealthGezondheidDieteticsVoedingsleerAscorbic acidBiological markerBleomycinCholesterolHorlicksOxygen radicalPlaceboProtein p21Ras proteinSanatogenAdultAgedAntioxidant activityCholesterol blood levelChromosome aberrationClinical trialComet assayConference paperControlled clinical trialControlled studyCrossover procedureDNA damageDose responseFemaleHumanHuman experimentLipid peroxidationLymphocyteMaleNormal humanOxidative stressPriority journalSex differenceVitamin blood levelVitamin supplementation
TNO Identifier
41673
Source
Pure and Applied Chemistry, 72(6), pp. 973-983.
Pages
973-983
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