Analysis of proteinaceous antinutritional compounds in soya oilseed products
bookPart
In the food and feed industry large amounts of grain legumes are used for the formulation of endproducts meant for human and animal nutrition. In particular the use of soybean (Glycin max) products as milk replacers in the animal diet and the problems related to antinutritional compounds present in soya are well described. Soya contains compounds like trypsin inhibitors, lectins, nonstarch polysaccharides, phytic acid, anti- and/or allergenic proteins. A special need exists for the identification and characterization of these factors. In this study, procedures have been developed for the detection of soya trypsin inhibitors, using both immunochemical and trypsin inhibitory activity methods, for the detection of soya lectins by both sandwich ezyme-linked immunosorbent assay (ELISA) and functional lectin immuno assay (FLIA), and for the detection of the apparent anti- and/or allergenicity by immunochemical methods such as ELISA and Westernblotting using anti-soya calf and human sera, mouse anti-allergen soya P34, and rabbit anti-soya glycinin and β-conglycinin antibodies.
TNO Identifier
37989
ISSN
00976156
Publisher
American Chemical Society
Source title
Immunoassays for residue analysis : food safety
Editor(s)
Beier, R.C.
Stanker, L.H.
Stanker, L.H.
Pages
219-230
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