A mini-promotor lacZ gene fusion for the analysis of fungal transcription control sequences

article


Punt, P.J.

Kuyvenhoven, A.

Hondel, C.A.M.J.J. van den
A system for the in vivo analysis of fungal transcription control sequences, based on a mini-promoter, was designed. The mini-promoter, providing all sequences necessary and sufficient for transcription initiation, was derived from the Aspergillus nidulans gpdA promoter region. Transcription initiation was not affected by the introduction of transcription control sequences directly upstream from the mini-promoter. Furthermore, the expression of the mini-promoter was not affected by wide-domain carbon or nitrogen control circuits. Using the mini-promoter vector, a previously identified upstream activating sequence from the A. nidulans gpdA gene was further characterized.
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β-galactosidaseCarbon regulationGPD boxLacZ reporter geneNitrogen regulationRecombinant DNATargeted single-copy integrationTranscription start point(s)GalactosidaseAspergillus nidulansGene expressionGene fusionGenetic transcriptionNitrogen balanceNonhumanPlasmidPriority journalPromoter regionReporter geneTranscription initiationAspergillus nidulansBase SequenceCloning, MolecularGene Expression Regulation, FungalGenetic VectorsGlyceraldehyde-3-Phosphate DehydrogenasesLac OperonMolecular Sequence DataOligodeoxyribonucleotidesPromoter Regions (Genetics)Transcription, GeneticAspergillusEmericella nidulans
TNO Identifier
37794
Repository link
https://resolver.tno.nl/uuid:a94bbbf0-250b-46a1-99c3-9db04177dbfd
Source
Gene, 158, pp. 119-123.
Publisher
Elsevier
Pages
119-123
Files
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