Model description of bacterial 3-methylcatechol production in one- and two-phase systems
article
Pseudomonas putida MC2 produces 3-methylcatechol from toluene in aqueous medium. A second phase of 1-octanol may improve total product accumulation. To optimise the design of such a biphasic process, a process model was developed, both for one- and two-phase applications. The insights obtained by the model predictions showed the importance of different process parameters (like growth substrate concentration and partition coefficient) on growth of biomass, accumulation of 3-methylcatechol and processing time. For future applications, the process model can be used to ensure enough extraction capacity from aqueous to octanol phase. It is a useful tool to define the optimum process conditions, depending on the desired optimisation parameter: product concentration or processing time. Chemicals/CAS: 3 methylcatechol, 488-17-5; octanol, 111-87-5, 29063-28-3; toluene, 108-88-3; 1-Octanol, 111-87-5; 3-methylcatechol, 488-17-5; Catechols; Toluene, 108-88-3
Topics
3-methylcatechol productionBiphasic processModellingPseudomonas putida mc23 methylcatechol3-methylcatecholcatechol derivativeoctanoltolueneunclassified drugaqueous solutionarticlebiological modelbiomassbioreactorcell culturecell divisioncomparative studycomputer simulationcytologyextractiongrowth, development and agingisolation and purificationmetabolismmethodologymicrobiologynonhumanpartition coefficientphysiologypriority journalprocess designprocess modelPseudomonas putidaquality controlreaction optimizationreproducibilitysensitivity and specificity1-OctanolBioreactorsCatecholsCell Culture TechniquesCell DivisionComputer SimulationModels, BiologicalPseudomonas putidaQuality ControlReproducibility of ResultsSensitivity and SpecificityTolueneBacteria (microorganisms)PseudomonasPseudomonas putida
TNO Identifier
237355
DOI
https://dx.doi.org/10.1007/s00449-003-0326-0
ISSN
16157591
Source
Bioprocess and Biosystems Engineering, 26(1), pp. 11-17.
Pages
11-17
Files
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