Optimizing the generation of random amplified polymorphic DNAs in chrysanthemum
article
Many conditions of the RAPD reaction procedure may influence the result. This paper presents rapid detection of influential factors with a fractional factorial experiment. A more extensive study of these factors is also presented. Polymerase brand, thermal cycler brand, annealing temperature, and primer, are important factors in obtaining good DNA yields and optimal fragment patterns. Each primer has its optimal annealing temperature, and this is not correlated with the GC content of the primer. Optimal species-primer combinations have to be found by trial and error. © 1993 Springer-Verlag.
TNO Identifier
50086
ISSN
00405752
Source
Theoretical and Applied Genetics, 86(8), pp. 1033-1037.
Pages
1033-1037
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