Reoxygenation of tumours in 'sandwich' chambers

article


Reinhold, H.S.

Blachiewicz, B.

Berg-Blok, A.
The process of reoxygenation of tumour tissue was studied in the rhabdomyosarcoma BA1112 following single doses of 10 and 20 Gy of X-rays. The analytical system consisted of the evaluation of the fluorescence response of the hypoxia-induced conversion of NAD to NADH. The tumours were grown for this purpose in thin, transparent 'sandwich' chambers. The results indicate that, only after a dose of 29 Gy reoxygenation of the tumour tissue takes place. There was an indication of an increase during the first day postirradiation which was followed by a dip at 2 days and a 2nd peak at 3.5 days after a dose of 20 Gy.
Chemicals/CAS: NAD, 53-84-9
Topics

















animal experimentin vitro studymethodologyratrhabdomyosarcomatherapyX rayAnimalAnoxiaFemaleMethodsMicroscopy, FluorescenceNADOxygen ConsumptionRatsSarcoma, ExperimentalTime FactorsX-Rays
TNO Identifier
228645
Repository link
https://resolver.tno.nl/uuid:bf686441-549c-4e6a-9c8d-26677972cd3f
ISSN
02775379
Source
European Journal of Cancer and Clinical Oncology, 15(4), pp. 481-489.
Pages
481-489
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