Chemical modification of proteases for wool cuticle scale removal
article
Over the last few decades several enzymatic processes to improve properties of wool fabrics like felting tendency, shrink resistance, dyeing ability and handling characteristics have been described. Previous investigations into the use of proteases to hydrolyse the cuticles at the surface of wool fibres, resulted in high strength and weight losses. Therefore restriction of the enzyme activity to the wool surface or control of enzyme diffusion to the cortex cells is required. To change the diffusion behaviour of proteases in wool fibres, the soluble polymer PEG was covalently attached to a protease from Bacillus lentus. Modified enzymes with different molecular weights were compared. These modified enzymes retained up to 80% of their activity in the standard assay while hydrolysis of wool fibres was successfully restricted to cuticles, resulting in a 90% decrease in weight losses compared to non-modified enzymes. © 2004 Taylor & Francis Ltd. Chemicals / CAS: macrogol, 25322-68-3; proteinase, 9001-92-7
Topics
Anti-shrinkageBacillus lentus proteaseDiffusionEnzyme modificationPolyethylene glycolWool treatmentEnzymesFabricsFibersMolecular weightPolyethylene glycolsShrinkproofing (textiles)Enzymatic processesFelting tendencyHyfrolyseProteasesWoolmacrogolproteinasearticlechemical modificationchemical procedurescontrolled studyEggerthella lentaenzyme activityenzyme kineticsenzyme stabilityenzyme synthesishydrolysismolecular weightnonhumanwoolEnzymesFibersForming FabricsPolyethylene GlycolWoolBacillus lentus
TNO Identifier
237952
ISSN
10242422
Source
Biocatalysis and Biotransformation, 22(5-6), pp. 299-305.
Pages
299-305
Files
To receive the publication files, please send an e-mail request to TNO Repository.