Induction of erythropoietin responsiveness in vitro
bookPart
Mouse bone marrow contains cells which form erythroid colonies in semisolid cultures containing erythropoietin (EP). Several sub-populatins of erythroid progenitor cells can be detected by varying the EP-concentration and the incubation time of the cultures. These sub-populations differ in apparent sensitivity to EP, proliferative potential, cycling state, physical characteristics and sensitivity toward in vivo manipulation of erythropoiesis. In the presence of relatively high erythropoietin concentrations very large erythroid colonies can be detected around the 10th day of incubation which eventually contain reticulocytes and erythrocytes. The cells from which these colonies originate have been termed erythroid burst-forming units. Evidence is accumulating indicating that the cell-divisions of BFU-e preceding hemoglobinization of the bursts occur independent of EP. In this paper we will summarize this evidence, describe the detection of activities apparently controlling these first divisions and discuss the significance of the findings with respect to the earliest differentiation events in hemopoiesis.
TNO Identifier
354379
Publisher
Academic Press
Source title
Hematopoietic cell differentiation
Pages
109-118
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