Palmitate oxidation and cytochromes in human and rat heart
article
Palmitate oxidation rates were determined in mitochondria and homogenates of human and rat heart muscle. Oxidation rates based on the sum of the production of 14CO_2 and 14C-labeled acid-soluble products from [14C]palmitate were in good agreement with those calculated from the oxygen consumption during palmitate oxidation. With limited amounts of substrate palmitate was completely oxidized to CO_2, but with non-limiting palmitate concentrations acetyl-carnitine and citric acid cycle intermediates accumulated. Palmitate oxidation rates per mg protein were about equal for human and rat heart muscle. Palmitate oxidation rates by homogenates and mitochondria were about equal, when expressed on the basis of cytochrome c oxidase activity for each species, but they differed between rat and man. This difference is caused by the lower activity of cytochrome c oxidase in human heart mitochondria. Difference spectrophotometric measurements showed, however, higher concentrations of cytochrome aa_3 and c + c_1 in human heart mitochondria than in those of the rat.
Topics
TNO Identifier
288179
Source
Journal of Molecular and Cellular Cardiology, 11(12), pp. 1245-1252.
Pages
1245-1252
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