Erythropoietin-independent regeneration of erythroid progenitor cells following multiple injections of hydroxyurea
article
It was shown previously that colony formation in vitro by early erythroid progenitor cells (BFUe) requires sequential stimulation with a specific glycoprotein termed BFA and erythropoietin (EP). The action exerted by BFA was characterized as induction of proliferation in BFUe resulting after several cell divisions in EP-responsive progeny. The present study is directed at detection of EP-independent regulation of erythroid progenitor cells in vivo. Hemopoietic regeneration was induced by multiple administrations of hydroxyurea (HU). The femoral regeneration patterns of hemopoietic stem cells (CFUs), granulocyte/macrophage progenitor cells (CFUgm) and erythroid progenitor cells (BFUe, day 3 BFUe and CFUe) were studied in hypertransfused mice in comparison to nontransfused controls. The results show that (1) the phase of exponential regeneration of none of the cell populations studied is affected by hypertransfusion; (2) each of these cell populations exhibit a distinct regeneration pattern, indicating that they behave as separate functional entities; and (3) the 3 erythroid cell populations are suppressed by hypertransfusion in the post-exponential phase of regeneration in contrast to CFUs and CFUgm. The results support a two-regulator model of erythropoiesis.
Chemicals/CAS: hydroxyurea, 127-07-1; iron 59, 14596-12-4; Erythropoietin, 11096-26-7; Glycoproteins; Hydroxyurea, 127-07-1
Chemicals/CAS: hydroxyurea, 127-07-1; iron 59, 14596-12-4; Erythropoietin, 11096-26-7; Glycoproteins; Hydroxyurea, 127-07-1
Topics
HydroxyureaIron 59RadioisotopeAnimal experimentBlood and hemopoietic systemErythroblastErythroid cellErythropoiesisErythropoietic stem cellMousePrecursor cellRegenerationThymidine h 3AnimalCell differentiationCell divisionCell survivalErythrocytesErythropoietinGlycoproteinsHematopoietic stem cellsHydroxyureaMice
TNO Identifier
228782
ISSN
0008-8730
Source
Cell and Tissue Kinetics, 13(5), pp. 505-517.
Pages
505-517
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