Isolation and culture of Kupffer cells from human liver. Ultrastructure, endocytosis and prostaglandin synthesis
article
Kupffer cells and other sinusoidal cells were isolated after perfusion and incubation with pronase and collagenase of pieces of liver tissue obtained from organ donors. The resulting cell preparations contained endothelial cells, Kupffer cells and fat-storing cells as well as considerable numbers of leucocytes. Attempts to purify the different sinusoidal cell types by density centrifugation and centrifugal elutriation were successful only for Kupffer cells. Kupffer cells, in contrast to endothelial cells and fat-storing cells, could be kept in maintenance culture for at least 5 days. Cultured Kupffer cells were active in the endocytosis of foreign substances, such as colloidal carbon, latex beads, horseradish peroxidase and bacterial endotoxin. The cultured Kupffer cells synthesized and secreted considerable amounts of prostaglandins PGE2, PGF(2α), 6-keto-PGF(1α) and thromboxane B2. The production of prostaglandins was influenced by the presence of Escherichia coli endotoxin.
Chemicals/CAS: Endotoxins; Horseradish Peroxidase, EC 1.11.1.-; Prostaglandins
Chemicals/CAS: Endotoxins; Horseradish Peroxidase, EC 1.11.1.-; Prostaglandins
Topics
TNO Identifier
230578
ISSN
01688278
Source
Journal of Hepatology, 6(1), pp. 36-49.
Pages
36-49
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