Centrifugal elutriation : separation of living cells
bookPart
Centrifugal elutriation is finding a place among other cell separation methods as a very promising procedure for the gentle purification of cell preparations enriched for one specific cell type. Single cells in suspension are subjected to two opposite forces in the chamber of an elutriator rotor: the centrifugal sedimentation velocity and the streaming velocity of a liquid flowing through the chamber in the centripetal direction. The cells will remain in the chamber when the two opposite velocities are the same. By incremental increases in the flow rate of the medium - or decreases in the centrifugal force - distinct types of cells will be washed (elutriated) out of the rotor sequentially, primarily according to their size. One of the main advantages of centrifugal elutriation is that living cells can be rapidly separated in any sui table medium, thus making it possible to obtain clearly divided populations of intact and highly viable cells retaining their activities and functions and with high recoveries. The applications of this method include the separation of various classes of blood cells and liver sinusoidal cells.
TNO Identifier
355329
Publisher
Pergamon
Source title
Separation of cells and subcellular elements
Editor(s)
Peeters, H.
Place of publication
Oxford
Pages
55-59
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