Fat-storing cells of the rat liver. Their isolation and purification

article


Knook, D.L.

Seffelaar, A.M.

Leeuw, A.M.de
Fat-storing cells from the lobular area of the rat liver have been isolated by digesting the liver with pronase E and collagenase, and purified by Metrizamide density centrifugation and centrifugal elutriation. More than 70% of the cells in the final fraction were fat-storing cells. Per gram wet weight of liver, 3.1 ± 0.5 × 106 cells were isolated. The purified cells showed a well preserved ultrastructure and contained lipid droplets with a fluorescence characteristic of vitamin A. A HPLC technique demonstrated the presence of large quantities of retinol and retinyl palmitate in the isolated fat-storing cells. Chemicals/CAS: collagenase, 37288-86-1, 39433-96-0, 9001-12-1; Lipids; retinol palmitate, 79-81-2; Vitamin A, 11103-57-4
Topics


















collagenaseanimal experimentelectron microscopyhistologyliverliver cellpronase eratAnimalCell SeparationCell SurvivalCentrifugation, Density GradientChromatography, High Pressure LiquidCytoplasmFemaleLipidsLiverRatsVitamin A
TNO Identifier
229217
Repository link
https://resolver.tno.nl/uuid:746f1791-23a2-407e-a8a7-5f70bdf5f031
ISSN
00144827
Source
Experimental Cell Research, 139(2), pp. 468-471.
Pages
468-471
Files
To receive the publication files, please send an e-mail request to TNO Repository.