Defined leukocyte antigens of chimpanzees : use of chimpanzee isoantisera for leukocyte typing in man
article
Leukocyte samples from 9 chimpanzees were serologically typed for 12 human leukocyte antigens using human isoantisera as reagents. Polymorphism was demonstrable for 7 of those antigens while one antigen was present on all chimpanzee leukocyte samples tested. Five of the animals were used for reciprocal skin grafting. Sixteen out of 20 grafts were technically successful and graft survival times ranged from 10-14 days. The demonstrable leukocyte and erythrocyte incompatibilities between hosts and immunizing donors are presented but the available data are considered insufficient for proper evaluation of the importance of the various antigens for histocompatibility in chimpanzees. Isoantisera produced by these 5 animals after skin grafting and after a number of boosting injections with chimpanzee leukocytes were tested against chimpanzee and human leukocytes, using primarily the cytotoxicity test. A preliminary analysis of the specificities of these sera revealed that 3 of the animals produced high titered isoantisera of sufficient specificity to be used as typing reagents for certain human leukocyte groups. Practical aspects of "directed" immunizations of chimpanzees to obtain human typing sera are briefly discussed.
TNO Identifier
356129
Source
Transplantation, 5(4), pp. 624-642.
Pages
624-642
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