Absence of malignant transformation after weekly irradiation of a mouse spleen cell culture
article
A nonmalignant mouse cell line was developed, which can be used for experiments on carcinogenesis. Special precautions were taken during the entire culture period to avoid the introduction of carcinogenic factors. Irradiation was tested in this in vitro system for its capacity to induce malignant transformations. The fact that exposure of the culture to weekly doses of 100, 200, or 300 rads of X radiation during a culture period of 1½ years, up to total doses exceeding 10,000 rads, did not produce malignant changes suggested that the irradiation had no direct carcinogenic effect on cells in vitro. The absence of a carcinogenic effect of X rays in vitro cannot be attributed to a peculiarity of the cell line, since cells of this line did turn malignant in a parallel experiment after introduction of a known cytotoxic serum.
TNO Identifier
288234
Source
Journal of the National Cancer Institute, 37, pp. 655-661.
Pages
655-661
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