Blood transfusions and changes in humoral and cellular immune reactivity in rhesus monkeys. Possible predictive value for kidney allograft prognosis
article
Pretreatment blood transfusions and matching for DR antigens are both procedures with a positive effect on kidney allograft prognosis. However, in both man and the monkey, a beneficial influence is seen in some but not all recipients; this leads to a so-called bimodal distribution of survival times. Therefore, in order to monitor the influence of transfusions on the immune responsiveness of kidney allograft recipients and to attempt to explain the phenomenon of bimodality of survival times, several techniques were used during the period in which transfusions were given to rhesus monkeys selected for kidney transplantation. Transfusions induced cytotoxic antibodies against a proportion of the lymphocyte samples tested (T + B and/or B cells) in most of the animals. Long-term surviving recipients had more frequent, earlier, and broader antibody responses than animals with short survival times, while antibodies directed against the specific kidney donor did not adversely affect the graft prognosis. When mixed lymphocyte culture (MLC) reactivities between host and donor before and after the transfusion period were compared, it was possible to predict to some extent the eventual fate of an allograft: increased MLC responsiveness predicted relatively short survival times, decreased MLC responsiveness relatively long ones. In vitro responsiveness to the mitogen phytohemagglutinin (PHA) was strongly reduced after transfusion in all animals; hence, no correlation was found with recipients' survival times. In vitro responses to other mitogens (pokeweed mitogen (PWM) and concanavalin A (Con A)) and to microbial antigens (vaccinia virus and purified protein derivative (PPD)) were not changed by transfusions. Finally, the impression was gained that blood transfusions could change the responsiveness to dinitrochlorobenzene (DNCB) in a conventional skin test performed just prior to kidney allografting. Interestingly, no short-term surviving animals were found among the nonresponders to DNCB. If confirmed, this test may have predictive value for a recipient's responsiveness also toward a kidney allograft.
Chemicals/CAS: 1 chloro 2,4 dinitrobenzene, 25567-67-3, 97-00-7; Concanavalin A, 11028-71-0; Dinitrochlorobenzene, 97-00-7; Histocompatibility Antigens Class II; HLA-DR Antigens; Phytohemagglutinins; Pokeweed Mitogens
Chemicals/CAS: 1 chloro 2,4 dinitrobenzene, 25567-67-3, 97-00-7; Concanavalin A, 11028-71-0; Dinitrochlorobenzene, 97-00-7; Histocompatibility Antigens Class II; HLA-DR Antigens; Phytohemagglutinins; Pokeweed Mitogens
Topics
1 chloro 2,4 dinitrobenzeneanimal experimentblood and hemopoietic systemblood transfusioncellular immunityhumoral immunitykidneykidney transplantationmonkeynonhumanAnimalAntibody FormationBlood TransfusionConcanavalin ADinitrochlorobenzeneFemaleGraft RejectionHistocompatibility Antigens Class IIHistocompatibility TestingHLA-DR AntigensImmunity, CellularKidney TransplantationLymphocyte ActivationLymphocyte Culture Test, MixedLymphocytesMacaca mulattaMalePhytohemagglutininsPokeweed MitogensPrognosisSkin TestsSupport, Non-U.S. Gov't
TNO Identifier
229379
ISSN
00411337
Source
Transplantation, 35(2), pp. 150-155.
Pages
150-155
Files
To receive the publication files, please send an e-mail request to TNO Repository.