Light scattering properties of pluripotent and committed hemopoietic stem cells

article

Engh, G. van den

Visser, J.
Mouse bone marrow cells were separated according to their light scattering properties in a light-activated cell sorter. The intensity of the scattered light was measured in a forward direction (FLS) and perpendicular to the incident light (PLS). These two parameters were found to give an indication of cell size and shape. The fractions containing cells with different light scattering properties were assayed for CFU-s (pluripotent stem cells) content and for the incidence of three types of CFU-c (cells committed to granulocyte/macrophage differentiation). These measurements confirm previous size calculations based on sedimentation rate and density separation. During maturation, the different colony-forming units (CFU) show a gradual increase in PLS intensity. This suggests that the transition from CFU-s through to CFU-c 3 is accompanied by a morphological differentiation.
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blood and hemopoietic systembone marrow cellhematopoiesishistologyin vitro studyAnimalBone MarrowCell DifferentiationCell SeparationColony-Forming Units AssayGranulocytesHematopoietic Stem CellsLasersLymphocytesMacrophagesMiceScattering, RadiationThymus GlandTissue Culture
TNO Identifier
228714
Repository link
https://resolver.tno.nl/uuid:57bae4bc-94f5-4f16-ac8b-ab1ed767edac
ISSN
00015792
Source
Acta Haematologica, 62(5-6), pp. 289-298.
Pages
289-298
Files
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