Isolation of infectious C type oncornavirus from human leukemic bone marrow cells

bookPart




Nooter, K.

Aarssen, A.M.

Bentvelzen, P.

Groot, G. de

Pelt, F.G. van
The authors report the isolation of a C type oncornavirus from a child with generalized lymphosarcoma. The primary sarcoma was found in the tonsils. Later on, the peripheral blood proved to be involved. When still untreated for the leukemia bone marrow was taken and cultured in the presence of phytohemagglutinin (PHA). After 3 days of culture C type particles were found in the supernatant as detected by a millipore filter technique. Bone marrow was also seeded on top of dishes with XC cells. Large syncytia were found 4 days later, while in the control cultures only a few small syncytia with 4 or less nuclei could be observed. Electronmicroscopic examination of the XC cultures demonstrated the release of C type particles, while control cultures proved to be negative with this technique. Virus production, as determined by electron microscopy or reverse transcriptase assay and syncytium formation, persisted for five passages in the infected XC cells, but declined strongly thereafter.
Chemicals/CAS: Antigens, Viral
Topics




















cell culturechildelectron microscopyin vitro studyleukemia cellleukoviruslymphosarcomamajor clinical studyoncovirinaetheoretical studyvirus isolationvirus particleAntigens, ViralBone MarrowBone Marrow CellsCells, CulturedChildHumanLeukemiaLymphoma, Non-HodgkinRetroviridae
TNO Identifier
228090
Repository link
https://resolver.tno.nl/uuid:b46b51ac-a6db-455e-942a-59a2cdc3d978
Publisher
Karger
Source title
Comparative Leukemia Research, Bibliotheca Haematologica
Editor(s)
Clemmesen, J.
Yohn, D.S.
Place of publication
Basel
Pages
574-575
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