Differential behavior of human bronchial carcinoma cells in culture
article
A feeder layer culture system suited to grow carcinoma cells derived from solid human lung tumors was developed. This report deals with culturing of the four main histological types of lung carcinomas observed in 37 patients: 19 squamous cell, 6 adenocarcinomas, 7 small cell, and 5 large cell carcinomas. The cultures were initiated from 24 fresh human surgical specimens and from 14 human lung tumors grown as xenografts in nude mice. Three different patterns of behavior in culture were found to be characteristic for squamous cell, adenocarcinomas, and small cell carcinomas, respectively. The culture pattern presented by the primary cultures did not appreciably change after passaging in vitro for periods of up to 2 years, even after infinite cell lines were established. Cultures of large cell carcinoma showed one or more of these patterns. From these patterns cells could be cloned and subsequently cultured as separate stable lines. The system described facilitates the identification of specific types of human lung carcinomas almost immediately (within 1 h) after plating (Phase I) as well as during culture.
Topics
adenocarcinomacell culturecloningcultural anthropologyhumanhuman cellin vitro studylung adenocarcinomalung small cell cancerlung squamous cell carcinomapriority journalrespiratory systemsmall cell carcinomasquamous cellAdenocarcinomaAnimalCarcinoma, BronchogenicCarcinoma, Small CellCarcinoma, Squamous CellCell AggregationCells, CulturedHumanLung NeoplasmsMiceMice, NudeSupport, Non-U.S. Gov't
TNO Identifier
230367
ISSN
00085472
Source
Cancer Research, 47(12), pp. 3251-3258.
Pages
3251-3258
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