Further studies on mobilization of CFUs
article
Mobilization of CFUs from haemopoietic tissues into circulation was studied after injection of different bacterial lipopolysaccharides (LPS), zymosan, phytohaemagglutinin (PHA), concanavalin A (Con A), trypsin and di-isopropyl-fluorophosphate-inhibited trypsin. All bacterial LPS used gave an increase of CFUs in the peripheral blood at 1 h after i.v. injection. Some variation in activity could not be excluded. As with Salmonella typhosa LPS, zymosan gave an increase in circulating CFUs during the first few hr and a second peak a few days later. After injection of zymosan as well as S. typhosa LPS the second peak in the blood was accompanied by a large increase in CFUs numbers in the spleen. PHA gave an immediate mobilization of CFUs, but the mobilization after injection of Con A during the first few hr occurred more slowly. After injection of S. typhosa LPS, zymosan and PHA the blood C3 level was found to be depressed considerably. This might indicate that the complement system is involved in the early mobilization of CFUs. Dexamethasone, a synthetic hormone which has been reported to give sequestration of several cell types in the bone marrow, did not inhibit the early and late mobilization of CFUs which normally occurs after injection of S. typhosa LPS. Copyright © 1979, Wiley Blackwell. All rights reserved
Topics
Animal experimentBlood and hemopoietic systemBone marrowColony forming unitHematopoietic cellMouseAnimalClonogenic assayComparative studyComplement activationCytologyDrug effectHematopoietic stem cellMalePhysiologySalmonella typhiColony-Forming Units AssayComplement 3Complement ActivationConcanavalin ADexamethasoneHematopoietic Stem CellsMicePhytohemagglutininsSalmonella typhiComplement component C3, 80295-41-6Concanavalin A, 11028-71-0Dexamethasone, 50-02-2Dyflos, 55-91-4;Phytohemagglutinin, 9008-97-3Trypsin EC 3.4.21.4, 9002-07-7Zymosan, 58856-93-2, 68652-43-7, 9010-72-4Complement 3Concanavalin A, 11028-71-0Isoflurophate, 55-91-4Lipopolysaccharides
TNO Identifier
572708
ISSN
0960-7722
Source
Cell Tissue Kinetics, 12(3), pp. 257-267.
Pages
257-267
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