Suppression of CFU-s activity by rabbit-anti-mouse brain serum can be overcome by treatment with papain
article
The suppression of CFU-s activity which is observed after incubation with rabbit-antimouse brain sera (RAMB) can be reversed by incubating the antibody coated cells with papain. Papain treatment does not alter the CFU-s membrane since pre-treatment with the enzyme does not protect CFU-s from the lethal effect of RAMB. This technique should allow the use of fluoresceinated or otherwise labelled RAMB for the selection of CFU-s.
TNO Identifier
354148
Source
Experimental Hematology, 6, pp. 627-630.
Pages
627-630
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