Identification of CFU-s by scatter measurements on a light activated cell sorter
conference paper
Light scattered by cells allows information about cell size and cell morphology to be gathered. Experiments are presented in which the intensity of forward light scatter (FLS) angles between 2 to 25 degrees and at perpendicular light scatter (PLS) angles between 70 to 110 degrees of colony forming units-spleen (CFU-s) are measured in a FACS II light-activated cell sorter. The scattering properties are compared to those of known cell types. It is concluded that CFU-s are spherical cells, measuring 7.2 μm in diameter, that do not contain conspicuous cytoplasmic inclusions. Light scatter can be used to eliminate erythrocytes and dead cells from immunofluorescence measurements. Fluorescence measurement of CFU-s in comparison to all bone marrow cells after treatment with anti-H-2 and anti-Thy-I sera are presented.
TNO Identifier
353402
Publisher
Springer
Source title
Experimental hematology today
Editor(s)
Baum, S.J.
Ledney, G.D.
Ledney, G.D.
Place of publication
New York
Pages
19-26
Files
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