Electrophoretic mobility properties of murine hemopoietic cells in different stages of development
article
In vivo spleen colony forming cells (CFU(s)) and three developmental stages of in vitro granulocyte/macrophage colony forming cells (GM CFU(c) 1, 2 & 3) are characterized by their surface charge density using the free-flow electrophoresis technique. CFU(s) have a somewhat higher electrophoretic mobility than have GM CFU(c). GM CFU(c) 1, 2 & 3 appear to be identical in their mobility properties. Treatment with neuraminidase - by which negatively charged N-aceylneuraminic acid (NANA or sialic acid) groups are cleaved from membrane glycoproteins - changes the electrophoretic mobility of the various hemopoietic cells differentially. Neuraminidase treatment strongly reduces the electrophoretic mobility of CFU(s) and GM CFU(c) 1, has a moderate effect on the mobility of GM CFU(c) 2 and causes a relatively small reduction in the mobility of GM CFU(c) 3. It is suggested that the number of neuraminidase susceptible NANA groups on the cell surface decreases during early hemopoietic cell development. Chemicals/CAS: sialidase, 9001-67-6; Neuraminidase, EC 3.2.1.18
Topics
sialidaseanimal experimentblood and hemopoietic systembone marrow cultureelectrophoresisin vitro studyAnimalBone Marrow CellsCell MovementCell SeparationColony-Forming Units AssayElectrophoresisFemaleHematopoietic Stem CellsMaleMiceMice, Inbred C3HMice, Inbred C57BLNeuraminidaseSpleenSupport, Non-U.S. Gov't
TNO Identifier
229055
ISSN
0301472X
Source
Experimental Hematology, 9(4), pp. 431-443.
Pages
431-443
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