A technique for staining haemopoietic colonies in agar cultures

article


Bol, S.

Engh, G. van den

Visser, J.
Good results were obtained using a tetrazolium salt (2-p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride) for staining hemopoietic colonies in agar cultures. This technique is based on the capacity of viable cells to reduce colorless tetrazolium salts to water-insoluble red formozan, which forms intracellular precipitates. The time needed for enumeration of colonies may be reduced to one-third of that required in the conventional procedure. (Blum - Terre Haute, Ind.)
Chemicals/CAS: Agar, 9002-18-0
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bone marrowcell culturecolony formationcytologyhematopoiesisin vitro studymethodologyAgarAnimalBone Marrow CellsCell CountCell DivisionClone CellsHematopoiesisHematopoietic Stem CellsMiceStaining and Labeling
TNO Identifier
228211
Repository link
https://resolver.tno.nl/uuid:facfd315-72f2-47bf-81cc-838aaa0a983f
ISSN
0301472X
Source
Experimental Hematology, 5(6), pp. 551-553.
Pages
551-553
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