Attempts at morphological identification of the hemopoietic stem cell in rodents and primates
article
In mouse and primate hemopoietic suspensions a quantitative parallelism is evident between the functional HSC assays and a cell type whose morphologic characteristics have not been described before. The stem cell content, according to the functional HSC assays in the various hemopoietic suspensions tested, varied from 0.4-25%. Although the authors are aware that the correlative studies are not direct proof, these data strongly indicate that the cell which fulfils the morphologic criteria listed in this paper is the HSC. Now that a detailed description of the morphologic properties of the candidate stem cell is available, these cells can be enumerated in various abnormal states of hemopoiesis. Obviously, such analysis will be facilitated by concentrating these cells, e.g. with the density gradient technique.
Topics
bone marrowbone marrow celldensity gradient centrifugationelectron microscopyhematopoiesishematopoietic cellmousetheoretical studyAnimalBone MarrowBone Marrow CellsCell FractionationCell NucleusCentrifugation, Density GradientClone CellsComparative StudyCytoplasmGolgi ApparatusHaplorhiniHematopoietic Stem CellsHumanLymphocytesMethodsMiceMicroscopy, ElectronMitochondriaSpecies SpecificitySpleenStaining and LabelingThoracic Duct
TNO Identifier
227576
ISSN
0301472X
Source
Experimental Hematology, 1(1), pp. 36-45.
Pages
36-45
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