Increased cytogenetic damage in smokers deficient in glutathione S-transferase isozyme mu

article



Poppel, G. van

Vogel, N. de

Bladeren, P.J. van

Kok, F.J.
Reduced expression of the μ-isozyme of glutathione S-transferase (GST; EC 2.5.1.18) has been associated with increased lung cancer risk. We studied the association between GST-μ expression and DNA damage as measured by sister chromatid exchanges (SCE) in healthy male smokers. SCE levels were higher in the 71 GST-μ-deficient smokers compared to the 83 non-deficient smokers (5.24 versus 4.97 SCE/lymphocyte; P = 0.09). In smokers having high plasma cotinine levels (> median of 315 ng/ml), this μ-related difference was more pronounced (5.50 versus 4.97; P= 0.01), whereas it was absent in smokers having low cotinine levels (4.95 versus 4.97; P= 0.92). Increased cytogenetic damage in GST-μ-deficient heavy smokers may thus explain the association between GST-μ expression and lung cancer.
Chemicals/CAS: cotinine, 486-56-6; glutathione transferase, 50812-37-8; Glutathione Transferase, EC 2.5.1.18; Isoenzymes; Nicotine, 54-11-5
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MedicineGeneeskundePathologyAdultChromatography, GasComparative StudyDNA DamageEnzyme-Linked Immunosorbent AssayGlutathione TransferaseHumanIsoenzymesLymphocytesMaleNicotineSister Chromatid ExchangeSmokingSupport, Non-U.S. Gov't
TNO Identifier
63978
Repository link
https://resolver.tno.nl/uuid:e75274bd-1728-4de2-92af-be8f130c54ea
ISSN
01433334
Source
Carcinogenesis, 13(2), pp. 303-305.
Pages
303-305
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