Antigenic structure of murine mammary tumour viruses
article
Rabbit antisera against purified standard murine mammary tumour virus (MTV-S) precipitate the virions of four different virus strains in double immunodiffusion: MTV-S, -L, -P and -O. The sera detect eight soluble antigens of MTV-S and -O, but only five of -P and three of -L. The effect of trypsin, pepsin, HCl RNAase, phosphilipase C and acridine orange on the appearance of precipitation lines of MTV-S antigens was investigated. On the basis of these results, it could be concluded that two soluble trypsin-sensitive antigens are located inside the nucleoid. They seem to contain some RNA fragments which do not influence antigenicity. They are group specific. Three other soluble antigens are membrane-constituents. Phospholipase C seems to increase their reactivity in immuno-diffusion. They all contain a protein moiety which is essential for antigenicity. Two of the membrane antigens are involved in immunoprecipitation of the intact virion; one of them being group-specific. © 1973.
TNO Identifier
227443
ISSN
00142964
Source
European Journal of Cancer (1965), 9(8), pp. 567-572.
Pages
567-572
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