Improved determination of phytate by ion-exchange chromatography
article
An improved method for the ion chromatographic determination of phytie acid is presented. The method is based on a recently published chromatographic method combined with a treatment of the samples with EDTA. Separation and quantitation of phytie acid were performed on an anion-exchange column. Phytie acid was detected at 300 nm after reaction with a ferric salt in an in-line postcolumn derivatization. The improved method described here was compared with a recently published HPLC method without EDTA treatment and with a method using small single-use ion-exchange columns. It was found that the HPLC method without EDTA treatment of the samples results in values too low for phytie acid, whereas the method using small single-use ion-exchange columns gives values too high. The differences between the results of the three methods were statistically significant (p < 0.01). © 1991 American Chemical Society.
Topics
TNO Identifier
231692
ISSN
00218561
Source
Journal of Agricultural and Food Chemistry®, 39(10), pp. 1770-1772.
Pages
1770-1772
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