Factors interfering with cellular immunological responses to the murine mammary tumor virus in tumor-bearing mice
article
T-cell-enriched spleen cell fractions from BALB/c mice bearing virally induced mammary tumors become unresponsive with progressive tumor growth in the lymphocyte blastogenesis test, using purified mammary tumor virus (MTV) as an antigen. Reactivity can be restored by mild trypsinization or extensive washing of the cells. When reactive cells from mice immunized with MTV were incubated with the wash fluid of the initially unresponsive cells, MTV-specific blastogenesis was inhibited. Washings of normal cells had no such effect. Leukocytes from tumor-bearing mice also become unresponsive to MTV in the leukocyte adherence inhibition (LAI) assay. Trypsinization or extensive washing does not restore reactivity. However, such treatment of reactive cells from immunized animals abolishes a positive reaction in the LAI assay, using MTV as an antigen. The washings of spleen cells from tumor-bearing mice inhibit the reactivity of leukocytes from immunized animals. Sera from these mice could block the reaction in both cellular immunological tests. The inhibiting factors appeared early after the onset of tumor growth. By means of the Sepharose bead immunofluorescence assay it was established that wash fluid and sera with blocking activity contain MTV antigens as well as anti-MTV antibodies.
Chemicals/CAS: Antibodies, Viral; Trypsin, EC 3.4.21.4
Chemicals/CAS: Antibodies, Viral; Trypsin, EC 3.4.21.4
Topics
antigen antibody complexblocking factorbreast carcinomacancer growthcellular immunityimmune responsemicroorganismmousemouse mammary tumor oncovirusspleen cellt lymphocytetheoretical studyAnimalAntibodies, ViralFemaleFluorescent Antibody TechniqueImmune Adherence ReactionLeukocytesLymphocyte ActivationMammary Neoplasms, ExperimentalMammary Tumor Virus, MouseMiceSupport, U.S. Gov't, P.H.S.Trypsin
TNO Identifier
228214
ISSN
00207136
Source
International Journal of Cancer, 20(3), pp. 441-447.
Pages
441-447
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