Absence of an early increase of phospholipid phosphate turnover in mitogen stimulated B lymphocytes
article
An early increase in the phosphate incorporation in phosphatidylinositol (PI) was observed in CBA spleen suspensions only upon stimulation with the T cell mitogens phytohemagglutinin (PHA) and Concanavalin A. No specific increase in phosphatidylinositol phosphate turnover was found with lipopolysaccharide (LPS). In nude cells neither PHA nor LPS had any effect on the phosphate incorporation in PI. Corticosteroid resistant thymocytes stimulated with pokeweed mitogen showed a specific increase in PI phosphate incorporation in contrast to spleen cells from neonatally thymectomized ALS treated mice. It is concluded that B cells do not show an early increase in PI phosphate turnover and that different lymphocyte populations may show variations in their metabolic response towards a mitogenic stimulus. Chemicals/CAS: concanavalin A, 11028-71-0; nitrogen, 7727-37-9; phytohemagglutinin, 9008-97-3; pokeweed mitogen, 63231-57-2; Concanavalin A, 11028-71-0; Lectins; Lipopolysaccharides; Mitogens; Phosphates; Phosphatidylinositols
Topics
concanavalin acorticosteroidlipopolysaccharidelymphocyte antibodynitrogenphosphatidylinositolphospholipidphytohemagglutininpokeweed mitogenb lymphocytein vitro studylymphocytelymphocyte culturelymphocyte metabolismlymphocyte transformationmethodologymousephosphate p 32phosphatidylinositol phosphatet lymphocytetheoretical studythymectomythymidine c 14thymocyteAnimalB-LymphocytesConcanavalin ALectinsLipopolysaccharidesLymphocyte ActivationMiceMice, Inbred CBAMice, NudeMitogensPhosphatesPhosphatidylinositolsSpleenT-LymphocytesTime Factors
TNO Identifier
227758
ISSN
00088749
Source
Cellular Immunology, 14(3), pp. 429-434.
Pages
429-434
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