Increased transport of 2-aminoisobutyric acid in rat lymphocytes stimulated with concanavalin A
article
The transport of 2-aminoisobutyric acid has been studied in stimulated rat lymphocytes. The capacity to accumulate 2-aminoisobutyric acid increased twofold after a 5 min incubation with phytohaemagglutinin (PHA) and concanavalin A (conA). It reached a maximum level which was 6-8 times the level of the control culture after 24 h of incubation with PHA. The early increased transport induced by conA could not be attributed to a decreased efflux, increased exchange diffusion or passive diffusion but reflected an increased rate of active influx. Kinetic analysis showed an increase in the maximum velocity of uptake (Vmax) while no effect was observed on the apparent affinity (Km). The increased uptake was dependent on protein synthesis and only partly dependent on the generation of metabolic energy. It is concluded that concanavalin A has no direct effect on the binding of 2-aminoisobutyric acid to the carrier and on the mobility or the number of carriers. © 1973.
Chemicals/CAS: 2 amino 2 methylpropionic acid, 62-57-7; carrier protein, 80700-39-6; concanavalin A, 11028-71-0; cycloheximide, 642-81-9, 66-81-9; protein, 67254-75-5; puromycin, 53-79-2, 58-58-2; sodium, 7440-23-5; Aminoisobutyric Acids; Carrier Proteins; Concanavalin A, 11028-71-0; Culture Media; Cycloheximide, 66-81-9; Lectins; Proteins; Puromycin, 53-79-2; Sodium, 7440-23-5
Chemicals/CAS: 2 amino 2 methylpropionic acid, 62-57-7; carrier protein, 80700-39-6; concanavalin A, 11028-71-0; cycloheximide, 642-81-9, 66-81-9; protein, 67254-75-5; puromycin, 53-79-2, 58-58-2; sodium, 7440-23-5; Aminoisobutyric Acids; Carrier Proteins; Concanavalin A, 11028-71-0; Culture Media; Cycloheximide, 66-81-9; Lectins; Proteins; Puromycin, 53-79-2; Sodium, 7440-23-5
Topics
2 amino 2 methylpropionic acidcarrier proteinconcanavalin Acycloheximidelectinproteinpuromycinsodiumactive transportanimalarticlebiosynthesiscell countcell survivalculture mediumdiffusiondrug effectkineticslymphocytemalemetabolismratstimulationtimeAminoisobutyric AcidsAnimalBiological Transport, ActiveCarrier ProteinsCell CountCell SurvivalConcanavalin ACulture MediaCycloheximideDiffusionKineticsLectinsLymphocytesMaleProteinsPuromycinRatsSodiumStimulation, ChemicalTime Factors
TNO Identifier
227394
ISSN
00144827
Source
Experimental Cell Research, 76(1), pp. 63-72.
Pages
63-72
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