Effect of different doses of 3-methylcholanthrene on the localization of the 3-methylcholanthrene-inducible isoenzymes of cytochrome P450 within the centrilobular and periportal zones of the rat liver
article
Immunohistochemical staining techniques were used to investigate the localization of the 3-methylcholanthrene inducible isoenzymes (P450 IA1 and IA2) in the rat liver. The rats were induced with different doses of 3-methylcholanthrene, ranging from 2.5 to 25 mg/kg body weight. A heterogeneous induction pattern was observed with induction doses of 2.5; 5; 7.5 and 10 mg/kg body weight with the highest concentration of the isoenzymes around the central vein. With a dose of 25 mg/kg body weight, a homogeneous pattern was found. Induction with a dose of 15 mg/kg body weight resulted in an intermediate situation.
Topics
3 methylcholanthrenecytochrome p450 isoenzymeanimal cellanimal experimentarticledrug doseenzyme localizationhistochemistryintraperitoneal drug administrationlivernonhumanpriority journalratAnimalCytochrome P-450 Enzyme SystemDose-Response Relationship, DrugEnzyme InductionImmunohistochemistryIsoenzymesLiverMaleMethylcholanthreneRats
TNO Identifier
231176
ISSN
00062952
Source
Biochemical Pharmacology, 39(11), pp. 1703-1708.
Pages
1703-1708
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