The PIT method: an automated in vitro technique for drug toxicity testing
article
An automated in vitro technique for drug toxicity testing is described. Human tumor cells were cultured for 2 days in 96-well microtiter plates before the addition of serial dilutions of drugs. At day 5 the cultures were terminated by the addition of a solution containing propidium iodide, ink and triton X-100 (PIT). Triton X-100 lysed all cells, which were subsequently stained by the DNA specific fluorescing propidium iodide. The ink effectively quenched all background fluorescence. The plates were read on a computer controlled automated microscope with a photomultiplier. The results showed a linear relationship between cell number and fluorescence intensity. Reproducible dose-response curves were obtained for 6 drugs tested. A computer program calculated ID50 values, making use of adequate growth and no-growth controls. ?? 1987 Martinus Nijhoff Publishers.
Chemicals/CAS: octoxinol, 9002-93-1; propidium iodide, 25535-16-4; Antineoplastic Agents; Octoxynol, 9002-93-1; Polyethylene Glycols; Propidium, 36015-30-2
Chemicals/CAS: octoxinol, 9002-93-1; propidium iodide, 25535-16-4; Antineoplastic Agents; Octoxynol, 9002-93-1; Polyethylene Glycols; Propidium, 36015-30-2
Topics
anti-cancer drugsfluorescencein vitro techniquenew methodscreeningtoxicityantineoplastic agentdiagnostic agentmacrogol derivativeoctoxinolpropidium iodidearticlecell linecell survivaldrug effectfluorescencehumanneoplasmpathologyAntineoplastic AgentsCell LineCell SurvivalFluorescenceHumanNeoplasmsOctoxynolPolyethylene GlycolsPropidiumSupport, Non-U.S. Gov't
TNO Identifier
230407
ISSN
01676997
Source
Investigational New Drugs, 5(2), pp. 161-165.
Pages
161-165
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