The PIT method: an automated in vitro technique for drug toxicity testing
article
An automated in vitro technique for drug toxicity testing is described. Human tumor cells were cultured for 2 days in 96-well microtiter plates before the addition of serial dilutions of drugs. At day 5 the cultures were terminated by the addition of a solution containing propidium iodide, ink and triton X-100 (PIT). Triton X-100 lysed all cells, which were subsequently stained by the DNA specific fluorescing propidium iodide. The ink effectively quenched all background fluorescence. The plates were read on a computer controlled automated microscope with a photomultiplier. The results showed a linear relationship between cell number and fluorescence intensity. Reproducible dose-response curves were obtained for 6 drugs tested. A computer program calculated ID50 values, making use of adequate growth and no-growth controls. ?? 1987 Martinus Nijhoff Publishers.
Chemicals/CAS: octoxinol, 9002-93-1; propidium iodide, 25535-16-4; Antineoplastic Agents; Octoxynol, 9002-93-1; Polyethylene Glycols; Propidium, 36015-30-2
Chemicals/CAS: octoxinol, 9002-93-1; propidium iodide, 25535-16-4; Antineoplastic Agents; Octoxynol, 9002-93-1; Polyethylene Glycols; Propidium, 36015-30-2
Topics
anti-cancer drugs
fluorescence
in vitro technique
new method
screening
toxicity
antineoplastic agent
diagnostic agent
macrogol derivative
octoxinol
propidium iodide
article
cell line
cell survival
drug effect
fluorescence
human
neoplasm
pathology
Antineoplastic Agents
Cell Line
Cell Survival
Fluorescence
Human
Neoplasms
Octoxynol
Polyethylene Glycols
Propidium
Support, Non-U.S. Gov't
TNO Identifier
230407
DOI
https://dx.doi.org/10.1007/BF00203541
ISSN
01676997
Source
Investigational New Drugs, 5(2), pp. 161-165.
Pages
161-165
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