Identification of mononuclear cells in human blood: I. Qualitative and quantitative data on surface markers after formaldehyde fixation of the cells
article
The technical details of a fixation procedure with formaldehyde which was applied in a direct membrane immunofluorescence technique to mononuclear cells from normal human blood are described. After separation of the cells with Ficoll-Isopaque according to Boyum (1963) they were washed and fixed with 0.04% formaldehyde in PBS for 10 min and washed again. This cell suspension can be stored at 4??C for at least 24 hr and the slides prepared from them at -20??C for at least some months. In practice, this fixation procedure not only appeared to be effective in the preservation of cells but also showed a number of additional advantages, such as the short handling period, including the fixation procedure and the avoidance of loss of cells. Moreover, true B lymphocytes, as defined by the synthesis of immunoglobulins and the incorporation of these molecules into their cell membrane, are recognized convincingly.
Chemicals/CAS: Formaldehyde, 50-00-0; Immunoglobulins, Surface
Chemicals/CAS: Formaldehyde, 50-00-0; Immunoglobulins, Surface
Topics
TNO Identifier
228819
ISSN
00099104
Source
Clinical and Experimental Immunology, 41(3), pp. 559-566.
Pages
559-566
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