The effect of age on protein synthesis by isolated liver parenchymal cells
article
The protein synthesizing capacity of liver parenchymal cells isolated from 3-, 12-, 24-, 31- and 36-mth-old rats was determined by the incorporation of 14C-leucine. Conditions for optimum protein synthesis included the use of an enriched medium (modified Waymouth's MB 752/1) and cell suspension concentrations ranging from 0.25 to 4 x 106 cells/ml medium. The cells were incubated with a dose of 6 μmol leucine/ml medium for 2 hr at 37°C under an atmosphere of 95% O2 and 5% CO2. With parenchymal cell isolated from 3-mth-old rats, a leucine incorporation rate of 14.4 nmol leucine/hr/106 cells was found. The capacity of the parenchymal cells to synthesize protein, decreased between 3 and 12 mth, remained constant between 12 and 24 mth and increased between 24 and 36 mth. Degradation of newly synthesized proteins or reutilization of 14C-leucine did not occur during the incubation period. The ratio between albumin and total protein synthesis as a function of age was determined. This ratio did not change between 3 and 24 mth but there was a significant increase between 24 and 36 mth. The increase in total protein synthesis in late age may be due to a compensation by the liver for a more pronounced proteinuria, increased proteolysis or an accumulation of 'altered' proteins.
Chemicals/CAS: Leucine, 61-90-5; Proteins
Chemicals/CAS: Leucine, 61-90-5; Proteins
Topics
TNO Identifier
228254
ISSN
00476374
Source
Mechanisms of Ageing and Development, 6(4), pp. 293-304.
Pages
293-304
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