Extraction and fractionation of wheat flour proteins
article
Extraction of wheat flour with 1.5% sodium dodecyl sulphate (SDS) solution dissolved 65–67% of the total flour nitrogen. The SDS‐insoluble proteinaceous material was separated into glycoproteins‐I, II and III by ultracentrifugation. Part of the SDS‐soluble proteinaceous material was precipitated by addition of ethanol and separated into glycoproteins‐IV, glutenins and globulins. The rest of the dissolved proteinaceous material was separated into glycoproteins‐V, gliadins, glycoproteins‐VI, and albumins by (NH4)2SO4‐precipitation and gel filtration chromatography
TNO Identifier
547882
Source
Journal of the Science of Food and Agriculture, 33, pp. 1117-1128.
Pages
1117-1128
Files
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