Plasminogen activator inhibitor 1 (PAI-1) antigen levels in human plasma samples
article
A new sandwich-type enzyme immunoassay for PAI-1 was evaluated for measurements in platelet-poor plasma samples. PAI-1 antigen in 26 healthy volunteers varied considerably from 29% to 233% of pooled normal plasma and correlated well with PAI activity (r = 0.89). A positive correlation was also found with t-PA antigen (r = 0.86). PAI-1 antigen, as measured in 10 volunteers, gradually decreased during the day from 92% at 09.00 h to 47% at 15.00 h. PAI-1 antigen in blood sera was on average 13 times higher than in platelet-poor plasma samples of the same individuals. This was ascribed to the high PAI-1 content of platelets. Analysis of plasma samples of 19 selected patients with venous thrombosis and high PAI activity also showed elevated PAI-1 antigen levels, up to 800%. It is concluded that the enzyme immunoassay might be useful to determine specifically PAI-1 levels in plasma samples. Time of day and platelet release are critical factors in blood collection procedures.
Chemicals/CAS: plasminogen activator inhibitor, 105844-41-5
Chemicals/CAS: plasminogen activator inhibitor, 105844-41-5
TNO Identifier
230613
ISSN
02689499
Source
Fibrinolysis, 2(2 Suppl.), pp. 79-82.
Pages
79-82
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