New methods for the detection of genetic variation.

article
Vijg, J.
The possibility to detect variations in the genetic material is limiting to measure DNA sequence variations occurring at low frequency, such as somatic mutations responsible for the generation of diversity in the immunoglobulin genes. In order to study mutations, a system has been developed using transgenic mice in which mutant genes are rescued and selected among non mutant genes by the phage lambda as shuttle vector. Moreover, it has been developed a two-dimensional DNA fingerprinting method based on the capacity to separate digested DNA on the size and base sequence.
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animalbacteriophage lambdaDNA fingerprintingDNA vectorgenetic selectiongenetic variabilitygeneticsmousemutagen testingmutationnucleotide sequencereviewtransgenic mouseAnimalBacteriophage lambdaDNA FingerprintingDNA Mutational AnalysisGenetic VectorsMiceMice, TransgenicMutagenicity TestsMutationSelection (Genetics)Variation (Genetics)
TNO Identifier
231373
Repository link
https://resolver.tno.nl/uuid:0e832325-4328-4984-a253-db43696114e0
ISSN
00212571
Source
Annali dell'Istituto Superiore di Sanita, 27(1), pp. 123-126.
Pages
123-126
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