Comparison of the oxygen enhancement ratio for γ ray induced double strand breaks in the DNA of bacteriophage T7 as determined by two different methods of analysis
article
Bacteriophage T7 was irradiated in a protecting medium under nitrogen and oxygen with 60Co gamma rays. Double strand breaks were measured by sucrose gradient sedimentation and by boundary sedimentation analysis. Both methods showed that the presence of oxygen during irradiation enhanced the production of double strand breaks. This is at variance with a recent report which suggests that boundary sedimentation analysis does not show an effect of oxygen. The discrepancy must be ascribed to differences in interpretation of the sedimentation data.
Chemicals/CAS: cobalt 60, 10198-40-0; nitrogen, 7727-37-9; oxygen, 7782-44-7; cobalt, 7440-48-4; Cobalt Radioisotopes; DNA, Viral; Oxygen, 7782-44-7
Chemicals/CAS: cobalt 60, 10198-40-0; nitrogen, 7727-37-9; oxygen, 7782-44-7; cobalt, 7440-48-4; Cobalt Radioisotopes; DNA, Viral; Oxygen, 7782-44-7
Topics
Cobalt 60NitrogenOxygenRadioisotopeCobaltVirus DNABacteriophageDNA strand breakageGamma radiationIn vitro studyMethodologyMicroorganismOxygen enhancement ratioTheoretical studyColiphageComparative studyDensity gradient centrifugationRadiation exposureRadiation responseCentrifugation, Density GradientCobalt RadioisotopesColiphagesComparative StudyDNA, ViralGamma RaysOxygenRadiation Effects
TNO Identifier
227853
ISSN
09553002
Source
International Journal of Radiation Biology, 27(5), pp. 437-446.
Pages
437-446
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