Contribution of various types of damage to inactivation of bacteriophage PM2 DNA in frozen solution by gamma radiation

article

Schans, G.P. van der

Bleichrodt, J.F.
When biologically active double stranded circular DNA of the bacteriophage PM2 in a solution of nutrient broth is irradiated at - 196°C by 60Co gamma rays, about 85% of the inactivation is due to nucleotide damage, i.e. damage in the nucleotides not associated with a chain break. Double strand breaks contribute some 10% to lethality and single strand breaks only a minor fraction if at all. About 10% of the nucleotide damage that is lethal if present in single stranded DNA is lethal in the double stranded PM2 DNA. These results do not show large differences from those obtained previously for irradiation of PM2 DNA under conditions of indirect effect.
Chemicals/CAS: DNA, 9007-49-2; Cobalt Radioisotopes; DNA, Circular; DNA, Viral; Nucleotides; Solutions
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DNABacteriophageDNA strandGamma radiationIn vitro studyMethodologyTemperatureTheoretical studyBacteriophagesCentrifugation, ZonalCobalt RadioisotopesDNA, CircularDNA, ViralFreezingGamma RaysNucleotidesPseudomonasRadiation GeneticsSolutions
TNO Identifier
227709
Repository link
https://resolver.tno.nl/uuid:256bdd6c-c3f4-44db-9649-113339814668
ISSN
09553002
Source
International Journal of Radiation Biology, 26(2), pp. 121-126.
Pages
121-126
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