Influence of oxygen on the induction of mutations in bacteriophage phiX174 by ionizing radiation
article
Several mutants of bacteriophage phiX174 having nonsense codons in different cistrons are reverted with a higher efficiency to pseudo wild type particles, i.e. particles able to propagate in suppressorless host bacteria, when irradiated with γ-rays in the presence of oxygen than when irradiated under anoxic conditions. For an ochre mutant of phiX174 it was found that the radiation induced conversion into an amber mutant, which corresponds with a transition of adenine in the parental DNA into guanine in the progeny DNA, is enhanced by oxygen. Differences between amber mutants of more than two hundred fold in the efficiency of induction of pseudo wild types show that the local environment of the amber codon in the phage particle exerts a great influence on the induction.
Chemicals/CAS: oxygen, 7782-44-7; Oxygen, 7782-44-7
Chemicals/CAS: oxygen, 7782-44-7; Oxygen, 7782-44-7
Topics
TNO Identifier
227692
ISSN
09553002
Source
International Journal of Radiation Biology, 25(5), pp. 505-512.
Pages
505-512
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