Quantitation of plasma levels of tetranectin - Effects of oral contraceptives, pregnancy, treatment with L-asparaginase and liver cirrhosis
article
Tetranectin is a tetrameric protein that binds to kringle 4 of plasminogen. Increase of electrophoretic mobility of the otherwise slowly migrating tetranectin in the presence of ethylenediaminetetraacetate was used to develop a reproducible electroimmunoassay to quantify plasma levels. Plasma levels in normals were found within narrow limits of 100 ± 16 (SD)%, (100% = 0.15 μmol/l). There was no difference between males and females, smokers and non-smokers, and there were no significnt changes with age from 20 to 49 years. Patients with severe liver cirrhosis showed a large variation in plasma tetranectin levels but no systematic or average reduction, in contrast to strong reductions in plasma levels of other proteins. Patients treated with L-asparaginase showed a gradual reduction in time in plasma levels of various proteins, though tetranectin showed no significant reduction. It is concluded that tetranectin can be assayed reproducibly in plasma and has a well regulated plasma level. This level is not sensitive to conditions with reductions in synthesis of many proteins, such as during cirrhosis of the liver and during L-asparaginase therapy. The reductions in plasma levels during the use of oral contraceptives and pregnancy indicate involvement of sex steroids in the metabolism of tetranectin. Chemicals/CAS: asparaginase, 9015-68-3; plasminogen, 9001-91-6; tetranectin, 109489-77-2; Asparaginase, EC 3.5.1.1; Blood Proteins; Contraceptives, Oral; tetranectin, 109489-77-2
Topics
asparaginaseplasminogentetranectinoral contraceptive agentplasma proteinadultblood levelclinical articleelectroimmunoassayfemalefibrinolysishumanliver cirrhosismetabolismpregnancypriority journaladolescentarticlebloodmalereference valueAdolescentAdultAsparaginaseBlood ProteinsContraceptives, OralFemaleHumanLiver CirrhosisMaleMiddle AgePregnancyReference Values
TNO Identifier
230802
ISSN
03406245
Source
Thrombosis and Haemostasis, 62(2), pp. 792-796.
Pages
792-796
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