Application of the extracellular α-amylase gene from Streptococcus bovis 148 to construction of a secretion vector for yogurt starter strains
article
Streptococcus thermophilus ATCC 19288, Lactobacillus delbrueckii subsp. bulgaricus T-11, and Lactococcus lactis subsp. lactis IL1403 were transformed with the α-amylase gene (amyA) from Streptococcus bovis 148 by using a wide host-range vector, and all the transformants secreted the α-amylase successfully. Since the promoter and the secretion signal of the amyA gene were functional in these strains, we constructed a secretion vector using the expression elements of amyA. Trials to secrete foreign enzymes in yogurt starter strains were performed using this novel secretion vector.
Topics
TNO Identifier
234100
ISSN
00992240
Source
Applied and Environmental Microbiology, 63(11), pp. 4593-4596.
Pages
4593-4596
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