Rescue of a transforming virus from a spontaneous nonproducing osteosarcoma in BALB/c mice

article


Bentvelzen, P.A.J.

Nooter, K.

Deys, B.F.
Cells from spontaneous osteosarcoma V793 that originated in a 19-month-old female BALB/c mouse were cultured. They did not produce a C-type oncovirus as determined by extracellular reverse transcriptase assay and cytoplasmic immunofluorescence. After cocultivation with Balb/3T3 cells chronically infected with a murine leukemia virus (MuLV), a focus-forming principle that transformed 3T3 cells, secondary BALB/c mouse embryo and WAG/Rij rat embryo fibroblasts were rescued. The transformation could be inhibited by antiserum to MuLV.
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transcriptaseanimal experimentbonecell transformationin vitro studymousemurine leukemia virusoncovirinaeosteosarcomaratvirus cell transformationAnimalCell LineCell Transformation, NeoplasticDefective VirusesGammaretrovirusHelper VirusesLeukemia Virus, MurineMiceOsteosarcomaSarcoma Viruses, MurineSarcoma, Experimental
TNO Identifier
228350
Repository link
https://resolver.tno.nl/uuid:3a9ea8a5-e9ea-403d-a941-0948925af45a
ISSN
00278874
Source
Journal of the National Cancer Institute, 60(2), pp. 401-403.
Pages
401-403
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