Transfection of mouse bone marrow cells with Rauscher virus proviral DNA

article



Nooter, K.

Berg, K. van den

Vries, J. de

Bentvelzen, P.
Mouse bone marrow cells were transfected with DNA isolated from Rauscher virus induced leukemic spleen cells. Successful transfection was manifested by virus production and transformation. Virus production by the transfected bone marrow cells was shown by the XC test. Injection of transfected cells into syngeneic hosts led to the development of erythroblastosis, probably due to the spread of virus reproduced by infectious proviral DNA. The transforming potential of the infectious viral DNA was demonstrated by the formation of erythroid colonies in vitro without the addition of erythropoietin to the culture medium.
Chemicals/CAS: DNA, Viral
Topics



























virus dnabone marrow celldiagnosiserythroblastosishistologyin vitro studymethodologymicroorganismmouseprovirusrauscher leukemia virusspleen celltheoretical studyAnimalBone MarrowBone Marrow CellsCell Transformation, NeoplasticCells, CulturedClone CellsCytopathogenic Effect, ViralDNA, ViralErythroblastsFemaleLeukemia, ExperimentalMiceRauscher VirusSpleenTransformation, Genetic
TNO Identifier
228026
Repository link
https://resolver.tno.nl/uuid:37b2750b-95d5-4470-a76c-76e3f20d6c83
ISSN
02775379
Source
European Journal of Cancer and Clinical Oncology, 12(8), pp. 633-637.
Pages
633-637
Files
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