Separation and identification of intact glucosinolates using direct coupling of High-Performance Liquid Chromatography with frit to fast-atom bombardment mass spectrometry
article
Intact glucosinolates are separated and identified in standard mixtures and in plant extract of Brussels sprouts by using the liquid chromatography frit to fast-atom bombardment mass spectrometry technique. The separation of the individual glucosinolates is achieved by using a conventional-bore high-performance liquid chromatography column with an aqueous ammonium acetate – acetonitrile gradient. The mandatory flow-rate reduction is achieved by using a pneumatic splitter. The determination limits are estimated to be below 20 μg/g in plant material for various glucosinolates using the sample pretreatment described. Copyright © 1989 Heyden & Son Ltd.
TNO Identifier
47957
Source
Rapid Communications in Mass Spectrometry, 3(4), pp. 102-106.
Pages
102-106
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